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was then almost wiped out by an epidemic. In 2001, an Indian gaur was born, cloned using genetic material from the Frozen Zoo and gestated in a domestic cow. Genetic rescue Although cloning isn’t perfect — the cloned Indian Gaur only survived for 48 hours — it’s a useful tool to help save endangered species, because it can increase genetic diversity. When the population of a species dwindles, the remaining animals are forced to inbreed and the genetic pool shrinks, further threatening survival. But the cloned Black-footed ferrets, for example, were born in 2020 from samples collected in 1988, which meant that their genetic profile was much more varied than the current population. “In a species of animals, the genetic diversity is what gives it its resilience, its ability to bounce back from natural catastrophes, virus attacks, disease attacks. That’s because if there are more different types of genetics in a species, there is a higher chance that some will survive,” explains Brendon Noble, a professor of regenerative medicine at the University of Westminster in London and chair of the board at The Frozen Ark, a UK-based animal cryobank. ‘Apathy is one of our biggest problems’: Ocean photographer Shawn Heinrichs wants to save the seas The Frozen Ark was founded in 2004, with a similar intent to the Frozen Zoo but with a different structure: rather than a single collection tied to one institution, it is a distributed network of over two dozen institutions such as zoos, museums and universities spread all over the world, each sharing its own collection and

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conservation efforts have sprouted around the world, and the tools that Benirschke didn’t yet have are now available. At the same time, the clock is ticking for many species at risk. Oliver Ryder is director of conservation genetics at the San Diego Zoo Wildlife Alliance and a geneticist at the Frozen Zoo. ‘An irreplaceable repository of very rare animals’ Since 1970, populations of mammals, birds, amphibians, reptiles and fish have fallen by 68% on average, according to the WWF Living Planet Report 2020. The report also states that as a result of loss of habitat due to human activities, one million species — animals and plants — are threatened with extinction over the coming decades and centuries. With the current rate of biodiversity loss, some scientists believe preserving samples from species that might not be here tomorrow is no longer a visionary endeavor, but a scientific must-do. Related story: How honey is helping to save the spectacled bear “As the effort built up, we realized that we were collecting an irreplaceable repository of very rare animals,” says Ryder. “Because we have cells in the Frozen Zoo, we can now apply new techniques and new technologies to extend our understanding, and learn more information that’s of direct relevance to preventing extinction in endangered species.” Since the Frozen Zoo was founded, Ryder says, many milestones have been reached in the field of genetics, starting with the cloning of the first animal — a sheep named Dolly — in 1996. Starting in 2001, four endangered species have been cloned using genetic material from the Frozen Zoo: the Indian Gaur, a humpbacked Asian wild ox; the Banteng, a Southeast-Asian species of cattle; Przewalski’s horse, once found throughout Mongolia and extinct in the wild until recently; and the Black-footed ferret, which was believed to be extinct in the wild until it resurfaced in 1981, but

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Back from the brink: How ‘frozen zoos’ could save dying species By Jacopo Prisco, Updated 0815 GMT (1615 HKT) March 31, 2022 Hide Caption 8 of 8 Photos: A ‘Frozen Zoo’ is helping to revive vanishing species The Frozen Zoo at San Diego Zoo’s Institute for Conservation Research contains samples from more than 1,200 species and subspecies, and is the largest repository of its kind. Four endangered species have so far been cloned using genetic material stored there. They include Przewalski’s horse, which is native to central Asia. In 2020 a baby horse known as “Kurt” was born in Texas, cloned using cells from the Frozen Zoo — the first successful cloning of the species. Hide Caption 1 of 8 ()When Kurt Benirschke started collecting skin samples from rare and endangered animals in 1972, he didn’t have a firm plan on what to do with them. As a researcher at the University of California San Diego, he believed that one day the tools would be developed to use them to save those animals. A few years later, he moved his collection to San Diego Zoo, and called it the Frozen Zoo. “Famously, there was a poster that hung above the Frozen Zoo with a quote that said, ‘You must collect things for reasons you don’t yet understand,'” says Oliver Ryder, a geneticist at San Diego Zoo and an early collaborator with Benirschke. “We felt that we were stewards of this growing collection that was going to have value to the future in ways we weren’t able to appreciate then.” Benirschke passed away in 2018, but his efforts are very much alive. Today, the Frozen Zoo is the world’s largest animal cryobank, with samples from over 10,500 individual animals from 1,220 species. For a long time, it was the only project of its kind; in recent years, however, similar

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